For comparisons of sense, antisense, and doublestranded RNA activity, equal masses of RNA were injected (that is, dsRNA was usedAdenine always bonds with thymine, and cytosine always bonds with guanine The bonding causes the two strands to spiral around each other in a shape called a double helix Ribonucleic acid (RNA) is a second nucleic acid found in cells RNA is a singlestranded polymer of nucleotidesHeitmeier et al, 1998;
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Double stranded rna is found in
Double stranded rna is found in-Double stranded RNA, also known as dsRNA, usually shows up in viruses and is somewhat unusual In viruses, it is a unique characteristic, and only a small number of viral families exhibit this trait RNA hybridization happens when one RNA strand combines, or hybridizes, with either another RNA strand or a deoxyribonucleic acid (DNA) strandThe base composition of an RNA virus was analyzed and found to be 1415% A, 140% U, 362% G and 357% C Would you conclude that the viral genetic material is
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The birth of long noncoding RNAs (lncRNAs) is closely associated with the presence and activation of repetitive elements in the genome The transcription of endogenous retroviruses as well as long and short interspersed elements is not only essential for evolving lncRNAs but is also a significant source of doublestranded RNA (dsRNA) The replication of poxvirus is unusual for a virus with doublestranded DNA genome (dsDNA) because it occurs in the cytoplasm, although this is typical of other large DNA viruses Poxvirus encodes its own machinery for genome transcription, a DNA dependent RNA polymerase, which makes replication in the cytoplasm possibleRibonucleic acid (RNA) is a molecule similar to DNA Unlike DNA, RNA is singlestranded An RNA strand has a backbone made of alternating sugar (ribose) and phosphate groups Attached to each sugar is one of four basesadenine (A), uracil (U), cytosine
RNA is doublestranded and contains sugar ribose and the nitrogencontaining base uracilA) a) double stranded DNA virus using DNA polymerase to replicate b) double stranded RNA virus using an RNA polymerase to replicate c) single stranded RNA virus using an RNA polymerase to replicate d) single stranded DNA virus using DNA polymerase to replicate e) none of the choices is correct Answer d Difficulty Medium Learning Objective LOMaggi et al, 00)
An isolate of the entomopathogenic fungus Beauveria bassiana was found to contain five doublestranded (ds) RNA elements ranging from 15 to more than 3 kbp The complete sequence of the largest dsRNA element is described here Analysis of the RdRp nucleotide sequence reveals its similarity to uncla Read mapping and counting Each replicate sample was sequenced by both nonstranded and stranded RNAseq The summaries for sequencing depth, mapping, and counting are shown in Fig 3 and listed in Additional file 1 Table S1For each sequenced library, there are over 60M pairedend reads (Fig 3a) available for alignment and gene quantification Overall, about I will keep it very very short and simple dsRNA is very critical in regulation of gene expression at translational level In our system we have specific miRNA (micro RNA) that are somewhat complementary to a specific mRNA and miRNAmRNA binding forms a double stranded molecule that is degraded by our system at a cellular level
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Although doublestranded (ds) RNA viruses had previously been identified in filamentous fungi, dsRNA viruses in yeast were discovered as determinants of the killer phenomenon in Saccharomyces cerevisiae and are now known to be associated with the presence of cytoplasmically inherited members of the totivirus family, which are frequently found Doublestranded RNA (dsRNA) is RNA with two complementary strands, similar to the DNA found in all cells, but with the replacement of thymine by uracil and the adding of one oxygen atom dsRNA forms the genetic material of some viruses (doublestranded RNA viruses) Doublestranded RNA, such as viral RNA or siRNA, can trigger RNA interferenceIn a double stranded RNA form, retroviruses infect a host cell with their genome, and then are reverse transcribed into double stranded DNA, with the DNA then integrated into the home cell genome When integrated into a host genome, a retrovirus is hard to detect and can lay dormant for prolonged periods, having no discernible effect on the host
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A doublestranded RNA genome isolated from a virus was found to contain 15% uracil What percentage of guanine should exist in this virus's genome?Retrovirus is an RNA virus They insert a copy of their genome into the DNA of host cells In its doublestranded RNA form, which then reverses transcribed into doublestranded DNA, and then gradually inserted into host cell The correct answer is B Answer verified by Toppr86 Words1 Page DNA is double stranded, while RNA is single stranded DNA is only found inside the nucleus and RNA can travel outside of the nucleus There is one type of DNA and 3 kinds of RNA DNA has a nitrogenous base named thymine that pairs with adenine In RNA instead of Thymine it has Uracil that pairs with adenine
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DNA double helix means that the twostranded structure of DNA structure is common knowledge, RNA's single stranded format is not as well known RNA can form into doublestranded structures, such as during translation, when mRNA and tRNA molecules pair DNA polymers are also much longer than RNA polymers;RNA interference (RNAi) is a genetic regulatory system that functions to silence the activity of specific genes RNAi occurs naturally, through the production of nuclearencoded premicroRNA (premiRNA), and can be induced experimentally, using short segments of synthetic doublestranded RNA (dsRNA)Doublestranded RNA viruses (dsRNA viruses) are a polyphyletic group of viruses that have doublestranded genomes made of ribonucleic acidThe doublestranded genome is used to transcribe a positivestrand RNA by the viral RNAdependent RNA polymerase (RdRp) The positivestrand RNA may be used as messenger RNA (mRNA) which can be translated into viral
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Double stranded RNA is a relevant immune stimulus with respect to SARSCoV2, an enveloped positive sense ssRNA virus (like flaviviruses, retroviruses) Once inside the cytoplasm sense RNA is read by ribosomes to make viral proteins and acting as a template to form an intermediate hybrid state with negative sense RNA to facilitate makingIt was found that only the two former enzymes cut across both strands, RNase TI cannot cleave double strands RNase 111 was shown to digest doublestranded RNA by a two step process an initial phase of specific cleavage is followed by random degradation In the first phase the enzyme exhibited a definiteDOUBLESTRANDED RNADEPENDENT PROTEIN KINASE Doublestranded (ds) RNA induces NFκB activation and iNOS expression in human astrocytes and murine RAW 2647 macrophages, suggesting a role of dsRNAdependent protein kinase (PKR) in iNOS induction by dsRNA (Auch et al, 04;
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In a double stranded RNA form, retroviruses infect a host cell with their genome, and then are reverse transcribed into double stranded DNA, with the DNA then integrated into the home cell genome When integrated into a host genome, a retrovirus is hard to detect and can lay dormant for prolonged periods, having no discernible effect on the hostIs it singlestranded or doublestranded?The 23m long human genome consists of
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最も人気のある！ double stranded rna is found in which virus RNAdependent RNA polymerase 6 (RDR6) is an essential component of RNAi, which plays an important role in the resistance against viruses in the model plants We found previously that rice RDR6 (OsRDR6) functioned in the defense against Rice stripe virus (RSV), and RiceRetrovirus Retrovirus is an RNA virus They insert a copy of their genome into the DNA of host cells In its doublestranded RNA form, which then reverses transcribed into doublestranded DNA, and then gradually inserted into host cell The correct answer is BThe base composition of a virus was found to be 11% A, 32% G, 18% U and 39% C It this a DNA or RNA virus?
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Double stranded form could assume a ZDNA structure b) If the singlestranded molecule has the sequence 5'(GATC)10, then its doublestranded form could assume an HDNA structure c) If the singlestranded molecule has the sequence 5'(CTGA)10, then its doublestranded form could assume a hairpin structure Doublestranded RNA (dsRNA) sometimes occurs It is similar to DNA, except thymine is replaced by uracil This type of RNA is found in some viruses When these viruses infect eukaryotic cells, the dsRNA can interfere with normal RNAAlthough the nonstandard G·T and C·T base pairs are normally not found in DNA, G·U base pairs are quite common in doublehelical regions that form within otherwise singlestranded RNA Two polynucleotide strands can, in principle, form either a righthanded or
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DNA and RNA differ in that A) DNA is singlestranded and contains the sugar deoxyribose and the nitrogencontaining base thymine;Here are many viruses with a doublestranded DNA genome that are known to infect mammals They are subdivided into seven virus families Hepadnaviridae, Polyomaviridae, Papillomaviridae, Adenoviridae, Herpesviridae, Poxviridae and Asfarviridae WiThe presence of Uracil shows that it is RNA The base composition is unequal, so it must be single stranded 2 Which of these DNA fragments will have a higher melting temperature?
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The structures of RNA and DNA are almost identical, so there is no reason why you can't have double stranded RNA Similarly DNA can be single stranded In fact, for DNA replication and transcription, the DNA strands must be separated dsRNA and ssDNA are most commonly found in New natural RNA function found Doublestranded RNA molecule UCSD scientist Steve Dowdy, who supplied this image, is working on doublestranded RNA drugs A new study indicates that double Summary Staufen homolog 2 is a member of the family of doublestranded RNA (dsRNA)binding proteins involved in the transport and/or localization of mRNAs to different subcellular compartments and/or organelles These proteins are characterized by the presence of multiple dsRNAbinding domains which are required to bind RNAs having double
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RNA or ribonucleic acid is a type of nucleic acid which is made up of ribonucleotides Generally, RNA is a singlestranded molecule, unlike DNA double helix In the Baltimore classification of viruses, Group III, Group IV and Group V include RNA viruses Some viruses have an ssRNA or singlestranded RNA genome Less common in nature is doublestranded RNA (dsRNA), which has two strands and resembles the wellknown DNA double helix It's found in some viruses, but for the past few decades, people have RNA interference has been used in the nematode Caenorhabditis elegans to manipulate gene expression Here we investigate the requirements for structure and delivery of the interfering RNA To our surprise, we found that doublestranded RNA was substantially more effective at producing interference than was either strand individually
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Doublestranded RNA (dsRNA) is an important regulator of gene expression in many eukaryotes It triggers different types of gene silencing that are collectively referred to as RNA silencing or RNAChanges in heterochromatin We suggest that this derepression leads to an increased accumulation of intracellular doublestranded RNA (dsRNA), with an attendant induction of innate immune responses that contribute to the neuroinflammation found in essentially all ageassociated neurodegenerative diseases
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